Zebravis Ppp2ca Antilichaam / Pp2a alfa

The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide. It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q567Y8

Zebrafish

An E.coli-derived zebrafish Ppp2ca recombinant protein (amino acids M1-L249) was used as the immunogen for the Zebrafish Ppp2ca antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Ppp2ca antibody is available for research use only.

After reconstitution, the Zebrafish Ppp2ca antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Ppp2ca antibody should be determined by the researcher.

IHC staining of FFPE zebrafish retina tissue with Ppp2ca antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.