Zebravis Smarca4a Antilichaam / Smarca4

Smarca4a in zebrafish is an ortholog of the human SMARCA4 protein also widely known as Brg1. The zebrafish protein shows strong sequence and functional conservation with the human enzyme, supporting the use of zebrafish as a model to study conserved mechanisms of chromatin remodeling and their roles in development and disease. Zebrafish also carries a related paralog named Smarca4b, which allows comparative analyses of partially overlapping and partially specialized functions within the genome duplication context of teleosts. Smarca4a is broadly expressed during zebrafish development, with higher levels in rapidly proliferating and differentiating tissues where large scale transcriptional reprogramming is required. Loss of Smarca4a function disrupts body patterning, organogenesis, and cell fate specification, underscoring its central role in controlling chromatin structure and transcription. Antibodies directed against zebrafish Smarca4a are valuable reagents for studying epigenetic regulation, transcriptional control, and developmental pathways. Typical applications include western blot to assess protein abundance, immunohistochemistry and immunofluorescence to map spatial expression in embryos and tissues, chromatin immunoprecipitation to define genomic binding sites, and immunoprecipitation based assays to characterize Swi Snf complex composition. Because zebrafish Smarca4a is an ortholog of human SMARCA4, findings obtained with these antibodies can inform research on human developmental disorders, cancer, and other diseases linked to defects in chromatin remodeling.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

Q7ZSY3

Zebrafish

E. coli-derived zebrafish Smarca4a recombinant protein (amino acids Q573-E775) was used as the immunogen for the Zebrafish Smarca4a antibody.

WB, IHC-P, IF

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Smarca4a antibody is available for research use only.

After reconstitution, the Zebrafish Smarca4a antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Smarca4a antibody should be determined by the researcher.

Nuclear

IHC staining of zebrafish Smarca4a protein using Zebrafish Smarca4a antibody, HRP-labeled secondary and DAB substrate. Smarca4a was detected in a paraffin-embedded section of zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.