Zebravis Tbx5a Antilichaam / Tbx5 / T-box eiwit 5a

Zebrafish Tbx5a is an ortholog of the human TBX5 protein, sharing significant sequence conservation and functional similarity. In both zebrafish and humans, Tbx5a regulates the formation of the heart's left ventricle and is involved in the proper patterning of the limbs during early development. Mutations in TBX5 in humans are associated with Holt-Oram syndrome, a disorder that leads to congenital heart defects and upper limb abnormalities. The conserved function of zebrafish Tbx5a makes it an ideal model for studying cardiac and limb development and for understanding the underlying genetic causes of congenital malformations. The use of a Zebrafish Tbx5a antibody allows researchers to monitor the expression of Tbx5a in various developmental stages, particularly in the heart and limb tissues. These antibodies are invaluable for applications such as western blot, immunohistochemistry, and immunofluorescence, enabling detailed study of Tbx5a expression and its role in patterning and organogenesis. A Zebrafish Tbx5a antibody is also useful for assessing the effects of genetic mutations or environmental factors on heart and limb development. Currently, no distinct isoforms of zebrafish Tbx5a have been identified, but its critical role in cardiac and limb development has been well documented. Using a high-quality Zebrafish Tbx5a antibody, researchers can explore the developmental pathways regulated by Tbx5a and gain insights into congenital diseases related to this transcription factor.

Western Blot: 0.5-1 µg/mL

Q9IAK8

Zebrafish

E. coli-derived zebrafish Tbx5a recombinant protein (amino acids M1-S492) was used as the immunogen for the Zebrafish Tbx5a antibody.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Tbx5a antibody is available for research use only.

After reconstitution, the Zebrafish Tbx5a antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Tbx5a antibody should be determined by the researcher.

Western blot analysis of Tbx5a protein using Zebrafish Tbx5a antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Predicted molecular weight ~55 kDa.