Anti-amphifysin/AMPH Antibody Picoband® fluoro550 Vervoeging

Amphiphysin is a protein that in humans is encoded by the AMPH gene. This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.

Neuronal acetylcholine receptor subunit alpha-5; CHRNA5; NACHRA5

AMPH

273

P49418

Human, Mouse, Rat

No cross-reactivity with other proteins

E.coli-derived human Amphiphysin/AMPH recombinant protein (Position: R58-K683) .

Flow Cytometry

Ligand-Gated Ion Channels, Neuroscience, Neurotransmission, Receptors / Channels

Immunogen affinity purified.

Liquid

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

1. David, C., Solimena, M., De Camilli, P. Autoimmunity in stiff-man syndrome with breast cancer is targeted to the C-terminal region of human amphiphysin, a protein similar to the yeast proteins, Rvs167 and Rvs161. FEBS Lett. 351: 73-79, 1994. 2. De Camilli, P., Thomas, A., Cofiell, R., Folli, F., Lichte, B., Piccolo, G., Meinck, H.-M., Austoni, M., Fassetta, G., Bottazzo, G., Bates, D., Cartlidge, N., Solimena, M., Kilimann, M. W. The synaptic vesicle-associated protein amphiphysin is the 128-kD autoantigen of stiff-man syndrome with breast cancer. J. Exp. Med. 178: 2219-2223, 1993. 3. Di Paolo, G., Sankaranarayanan, S., Wenk, M. R., Daniell, L., Perucco, E., Caldarone, B. J., Flavell, R., Picciotto, M. R., Ryan, T. A., Cremona, O., De Camilli, P. Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice. Neuron 33: 789-804, 2002.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

53054 MW

6

Neuronal acetylcholine receptor subunit alpha-5

Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Neuronal acetylcholine receptor subunit alpha-5

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.