Anti-Cdk1 / p34cdc2 Serine-Threonine Kinase Monoklonale Antilichaam (Kloon: A17.1.1)

Recognizes a 34kDa protein (cdk1), identified as p34cdc2 (a catalytic subunit of Maturation Promoting Factor) . Its epitope maps near the C-terminus of the protein and its core is thoμght to be LGTPNNEV (aa220-227 in murine cdc2) . It shows no cross reaction with cdk2 p32. It supports the kinase activity. p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is a serine/threonine kinase, which is activated by cyclin, by dephosphorylation of tyrosine residues. p34cdc2 is inactivated by a tyrosine kinase. This MAb reportedly inhibits the activation of p34cdc2 kinase by cyclins.

Cdc2, CDC28A, CDK1, CDKN1, CELL CYCLE CONTROLLER CDC2, Cell division control protein 2 homolog, Cell division cycle 2 G1 to S and G2 to M, Cell division protein kinase 1, Cyclin-dependent kinase 1, p34 Protein Kinase, P34CDC2

CDC2

983

P06493

Human, Mouse, Rat

C-Terminal 2/3rds of Xenopus cdc2 expressed in E.coli

A17.1.1

IF, IP, WB, IHC

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Inhibits activation of p34cdc2 kinase by cyclins; , Immunofluorescence (1-2ug/ml) ; Immunoprecipitation; Kinase Assay; Western Blot; , Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)

Mouse IgG2a, kappa