Anti-Crk-achtige proteïne CrkL Antibody Picoband® fluoro488 Vervoeging

CRKL (v-crk sarcoma virus CT10 oncogene homolog (avian) -like) also called ONCOGENE  CRKL, encodes a 303-amino acid polypeptide with a predicted molecular mass of 36 kD. While  CRKL  is not the human homolog of CRK, it is similar to protein-tyrosine kinases with SH2 and SH3 (src homology) domains. By fluorescence in situ hybridization,   the  CRKL  gene is localized to chromosome 22q11, centromeric of the chronic myelogenous leukemia breakpoint region. Senechal et al. (1996)   showed that  CRKL  becomes phosphorylated when overexpressed, activates Ras-dependent and JNK pathways, and transforms fibroblasts. The authors also found  CRKL  to be a substrate for the BCR-ABL tyrosine kinase, leading them to conclude thatCRKL  is a tyrosine kinase and an oncogene. Crkl  was required for normal cellular responses to Fgf8, including survival and migration, Erk activation, and target gene expression.

Crk-like protein; CRKL

CRKL

1399

P46109

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human CrkL, different from the related mouse and rat sequences by one amino acid.

Flow Cytometry

Oncoproteins/Suppressors, Proto-Oncogenes, Signal Transduction, Transcription

Immunogen affinity purified.

Liquid

May mediate the transduction of intracellular signals.

1. Guris, D. L., Duester, G., Papaioannou, V. E., Imamoto, A. Dose-dependent interaction of Tbx1 and Crkl and locally aberrant RA signaling in a model of del22q11 syndrome. Dev. Cell 10: 81-92, 2006. 2. Moon, A. M., Guris, D. L., Seo, J., Li, L., Hammond, J., Talbot, A., Imamoto, A. Crkl deficiency disrupts Fgf8 signaling in a mouse model of 22q11 deletion syndromes. Dev. Cell 10: 71-80, 2006. 3. Senechal, K., Halpern, J., Sawyers, C. L. The CRKL adaptor protein transforms fibroblasts and functions in transformation by the BCR-ABL oncogene. J. Biol. Chem. 38: 23255-23261, 1996.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

33777 MW

No cross reactivity with other proteins.

6

Crk-like protein

Nucleus. Cytoplasm.

Belongs to the CRK family.

Crk-like protein

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.