Anti-Cytochroom P450 2E1/CYP2E1 Antilichaam Picoband® fluoro488 Vervoeging

CYP2E1, also known as P450IIE1, is a member of the P450IIE subfamily which is ethanol-inducible. It has at least 1 gene which is mapped to 10q24.3-qter, and a second is likely in rat and in man. Both the rat and human proteins encoded by this gene contain 493 amino acids and calculated molecular masses of 56,634 and 56,916 daltons, respectively. In addition, genetic polymorphisms in the 5-prime flanking region of the human P450IIE1 gene affected its binding of transacting factor and changed its transcriptional regulation, which may lead to interindividual differences of microsomal drug oxidation activity. P450IIE1 is an important enzyme for the catalysis of the conversion of ethanol to acetaldehyde and to acetate in humans, and it is also involved in the metabolism of nitrosamines. Due to the possible correlation of P450IIE1 genes with malignancy, clinical studies of RFLP patterns of these genes in cancer may be useful.

Cytochrome P450 2E1;1.14.13.-;4-nitrophenol 2-hydroxylase;1.14.13.n7; CYPIIE1; Cytochrome P450-ALC; Cytochrome P450-J; Cyp2e1; Cyp2e, Cyp2e-1

CYP2E1

13106

Q05421

Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the N-terminus of mouse CYP2E1, identical to the related rat sequence.

Highest level in the liver and to a lesser extent in the kidney, with a higher level in the male kidney than in the female.

Flow Cytometry

Cancer, Cardiovascular, Cytochromes, Drug Metabolism, Lipases, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction

Immunogen affinity purified.

Liquid

Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. .

1. Nebert, D. W.; Adesnik, M.; Coon, M. J.; Estabrook, R. W.; Gonzalez, F. J.; Guengerich, F. P.; Gunsalus, I. C.; Johnson, E. F.; Kemper, B.; Levin, W.; Phillips, I. R.; Sato, R.; Waterman, M. R. : The P450 gene superfamily: recommended nomenclature. DNA 6: 1-11, 1987. 2. Song, B.-J.; Gelboin, H. V.; Park, S.-S.; Yang, C. S.; Gonzalez, F. J. : Complementary DNA and protein sequences of ethanol-inducible rat and human cytochrome P-450s: transcriptional and post-transcriptional regulation of the rat enzyme. J. Biol. Chem. 261: 16689-16697, 1987. 3. Hayashi, S.; Watanabe, J.; Kawajiri, K. : Genetic polymorphisms in the 5-prime-flanking region change transcriptional regulation of the human cytochrome P450IIE1 gene. J. Biochem. 110: 559-565, 1991.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

56805 MW

No cross reactivity with other proteins.

6

Cytochrome P450 2E1

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Cytochrome P450 2E1

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.