Anti-Ep-CAM / CD326 (Extracellulair Domain) (Epithelial Marker) Monoklonale Antilichaam (Kloon: EGP40/1798)

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1) ; ECS-1; Epidermal Surface Antigen 1; ESA1; FLOT2; Flotillin-2; Memb

TACSTD1

4072

P16422

Human

Recombinant human EpCAM protein fragment from extracellular domain (around aa77-202) (exact sequence is proprietary)

EGP40/1798

ELISA, FACS, IF, WB, IHC

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA) ; , Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Western Blot (1-2ug/ml) ; , Immunohistochemistry (Formalin-fixed) (0.5-2ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes)

Mouse IgG2b, kappa