Anti-ERK1 (T207) /ERK2 (T188) [geconserveerd], fosforspecifiek antilichaam

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The ERK1/2 (p44/42) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK), a MAP kinase kinase (MAPKK), and a MAP kinase (MAPK) . Multiple ERK1/2 MAPKKKs have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate ERK1 and ERK2 through phosphorylation of activation loop residues Thr-202/Tyr-204 and Thr-185/Tyr-187, respectively. ERK1/2 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases. Several downstream targets of ERK1/2 have been identified, including p90RSK and the transcription factor Elk-1.

Our ERK1 (T207) /ERK2 (T188) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and xenopus ERK1 (T207) /ERK2 (T188) and is antigen affinity purified. It is great for use in ELISA, WB.

Our ERK1 (T207) /ERK2 (T188) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus ERK1 (T207) /ERK2 (T188) and is antigen affinity purified. It is great for use in WB.

ERK, p42, p44, MAPK

MAPK1/2

P63085

Thr-188

Chicken, Human, Mouse, Rat, and Xenopus

Phospho-ERK2 (Thr-188) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Thr-188 in mouse ERK2. This sequence is conserved in human, rat, chicken, and fish ERK2, and is highly conserved in ERK1 (Thr-207), ERK5 (Thr-224), and ERK7 (Thr-180) .

ERK1 (T207) /ERK2 (T188)

Unconjugated

ELISA, WB

Cell Signaling, Kinase Signaling, Neurodegenerative Disease, Alzheimer's Disease, Neurogenesis and Development, Oncoproteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Phosphorylated

42/44

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:1000

The antibody detects 42 and 44 kDa* proteins corresponding to ERK1 (Thr-207) and ERK2 (Thr-188) on SDS-PAGE immunoblots of human A431 epithelial cells stimulated with calyculin A. It does not detect these ERK proteins in control cells or in blots treated with lambda phosphatase.

Western blots performed on each lot.

Rabbit

Liquid

IgG

Mouse

Quality Control: WB

AB_10704813