Anti-Giantin/GOLGB1 Antibody Picoband® fluoro488 Vervoeging

Giantin or Golgin subfamily B member 1 is a protein that in humans is encoded by the GOLGB1 gene. GOLGB1, also known as Giantin and Macrogolgin, is a 360 to 400 kDa member of the Golgin protein family. GOLGB1 is an integral component of the Golgi membrane that associates with the cytoskeleton. It may be involved in both forming intercisternal cross-bridges that mediate stacking in Golgi complexes, and in reorganizing the complexes after they fragment during mitosis. GOLGB1 has also been identified as an autoantigen in chronic rheumatoid arthritis, and in the autoimmune disease Sjoegren syndrome. Human GOLGB1 is 3259 amino acids (aa) in length, and over aa 1596-1702, shares 92% and 91% sequence identity with its mouse and rat orthologs, respectively.

Transforming growth factor beta activator LRRC32; Garpin; Glycoprotein A repetitions predominant; GARP; Leucine-rich repeat-containing protein 32; LRRC32; D11S833E

GOLGB1

2804

Q14789

Human

No cross-reactivity with other proteins.

E.coli-derived human Giantin/GOLGB1 recombinant protein (Position: L153-E950) .

Heart, placenta, skeletal muscle, kidney, lung and pancreas.

Flow Cytometry

Immune System Diseases, Immunology, Protein Trafficking, Signal Transduction, Signaling Pathway

Immunogen affinity purified.

Liquid

Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta. Able to outcompete LTBP1 for binding to LAP regulatory chain of TGF-beta. Controls activation of TGF-beta-1 (TGFB1) on the surface of activated regulatory T-cells (Tregs) . Required for epithelial fusion during palate development by regulating activation of TGF-beta-3 (TGFB3) (By similarity) .

1. Gross, M. B. Personal Communication. Baltimore, Md. 4/17/2014. 2. Lan, Y., Zhang, N., Liu, H., Xu, J., Jiang, R. Golgb1 regulates protein glycosylation and is crucial for mammalian palate development. Development 143: 2344-2355, 2016. 3. Linstedt, A. D., Hauri, H.-P. Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa. Molec. Biol. Cell 4: 679-693, 1993.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

Leucine rich repeat containing 32

Cell membrane. Single-pass type I membrane protein. Cell surface.

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.