Anti-LIMK1 (Thr-508) [LIMK2 (Thr-505), fosforspecifiek antilichaam

LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains. LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK. PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr-508 or Thr-505 residues in the activation loop, increasing LIMK activity. In addition, VEGF-induced stress fiber formation has been linked to p38-mediated activation of LIMK through MK-2 phosphorylation of Ser-323. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser-3 residue of cofilin. In addition, LIMKs may have a function in the nucleus. It has been shown that the nuclear localization of LIMKs can mediate suppression of Rac/Cdc42-mediated cyclin D1 expression. This effect of LIMKs was independent of cofilin phosphorylation and the regulation of actin dynamics.

Our LIMK1 (Thr-508) [LIMK2 (Thr-505] rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat LIMK1 (Thr-508) [LIMK2 (Thr-505] and is antigen affinity purified. It is great for use in ELISA, WB.

Our LIMK1 (Thr-508) [LIMK2 (Thr-505] rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat LIMK1 (Thr-508) [LIMK2 (Thr-505] and is antigen affinity purified. It is great for use in WB.

LIMK

LIMK1

P53667

Thr-508

Human, Mouse, and Rat

LIMK1 (Thr-508) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding threonine 508 in human LIMK1. This sequence is conserved in rat and mouse LIMK1, and has high homology to Thr-505 in human LIMK2.

LIMK1 (Thr-508) [LIMK2 (Thr-505]

Unconjugated

ELISA, WB

Cell Signaling, Cytoskeletal Regulation, Kinase Signaling, Oncoproteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Phosphorylated

72

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:1000

The antibody detects a 72 kDa* protein corresponding to the molecular mass of LIMK1 on SDS-PAGE immunoblots of activated mouse recombinant LIMK1, but does not detect this protein after lambda phosphatase treatment.

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_2265786