Anti-Melanoom Marker (MART-1 + gp100) Monoklonale Antilichaam (Kloon: DT101+ BC199 + HMB45)

This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A; PMEL17

MLANA & SILV

2315; 6490

Q16655 ; P40967

Human

Recombinant hMART-1 protein (DT101 & BC199) ; Extract of pigmented melanoma metastases from lymph nodes (HMB45)

DT101+ BC199 + HMB45

IHC

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)

Mouse IgG2b, kappa + Mouse IgG1, kappa