Anti-Neurofilament Zwaar, gefosforyleerd en niet-gefosforyleerd (pNF-H) Antilichaam

Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70 kDa with some variability across species. Antibodies to NF-L are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig's disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others. (Ref: uniprot.org)

Our Anti-Neurofilament heavy polypeptide , phosphorylated and non-phosphorylated (pNF-H) rabbit polyclonal primary antibody detects human, other mammals (predicted), and rat Neurofilament heavy polypeptide , phosphorylated and non-phosphorylated (pNF-H), and is whole serum. It is validated for use in IF, ICC, WB.

Our Anti-Neurofilament heavy polypeptide, phosphorylated and non-phosphorylated (pNF-H) rabbit polyclonal primary antibody detects human, other mammals (predicted), and rat Neurofilament heavy polypeptide, phosphorylated and non-phosphorylated (pNF-H), and is whole serum. It is validated for use in IF, ICC, WB.

NF-H; NFH; NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845

P12036

Human, Other Mammals (Predicted), and Rat

This antibody has been made against a rat NF-H construct containing most of the tandem KSP repeats expressed in and purified from E.coli.

Neurofilament heavy polypeptide , phosphorylated and non-phosphorylated (pNF-H)

Unconjugated

IF, ICC, WB

Whole serum

Lyophilized with sodium azide.

160 kDa

For research use only.

25°C (ambient)

Spin vial briefly before opening. Reconstitute with 50 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.

1:5000-1:10000

Species cross-reactivity includes human and rat. This antibody reacts with with phosphorylated NF-H at approx 200 kDa and non-phosphorylated NF-H at 160 kDa. Predicted to react with other mammals due to sequence homology.

1:500-1:1000

Rabbit

Lyophilized

Mixed

Rat