Anti-p130 Cas (Tyr-751), Fosfospecifiek Antilichaam

P130 Cas (Crk-associated substrate (CAS), breast cancer antiestrogen resistance 1 (BCAR1) ) is a docking protein containing multiple protein-protein interaction domains. The N-terminal SH3 domain functions as a molecular switch regulating CAS tyrosine phosphorylation, as it interacts with tyrosine kinases and phosphatases. The C-terminal Src binding domain contains a proline-rich motif that mediates interaction with the SH3 domains of Src-family kinases (SFKs) . Phosphorylation of this domain at Tyr-762 in rat (Tyr-668 in mouse) promotes this interaction. The p130 Cas central substrate domain is characterized by 15 tyrosines present in Tyr-X-X-Pro (YXXP) motifs, including Tyr-165, Tyr-249, and Tyr-410. When phosphorylated, most YXXP motifs are able to serve as docking sites for proteins with SH2 or PTB domains. In addition, phosphorylation of Tyr-751 (Tyr-653 in human) near the C-terminal caspase recognition site can attenuate caspase cleavage, while dephosphorylation occurs during apoptosis and may facilitate p130 Cas degradation.

Our p130 Cas (Tyr-751) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat p130 Cas (Tyr-751) and is antigen affinity purified. It is great for use in ELISA, WB.

Our p130 Cas (Tyr-751) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat p130 Cas (Tyr-751) and is antigen affinity purified. It is great for use in WB.

BCAR1, Cas

BCAR1

Q63767

Tyr-751

Human, Mouse, and Rat

Phospho-p130 Cas (Tyr-751) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 751 in rat p130 Cas. This rat sequence is the same as Tyr-653 in human p130 Cas and Tyr-657 in mouse p130 Cas.

P130 Cas (Tyr-751)

Unconjugated

ELISA, WB

Cell Adhesion Molecules, Cell Signaling, Kinase Signaling, Oncoproteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Phosphorylated

130

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:2000

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-p130 Cas (Tyr-762) peptide before affinity purification using phospho-p130 Cas (Tyr-751) peptide (without carrier) . The antibody detects a 130 kDa* protein corresponding to the molecular mass of phosphorylated p130 Cas on SDS-PAGE immunoblots of pervanadate treated human endothelial and A431 cells, but is not observed in control cells.

Western blots performed on each lot.

Rabbit

Liquid

IgG

Rat

Quality Control: WB

AB_2065562