Anti-SAP130 Antibody Picoband® 0,00647 Vervoeging

Histone deacetylase complex subunit SAP130 is an enzyme that in humans is encoded by the SAP130 gene. This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP) . The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF (II) 31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF (II) -containing complex) . These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

C-C motif chemokine 16; Chemokine CC-4; HCC-4; Chemokine LEC; IL-10-inducible chemokine; LCC-1; Liver-expressed chemokine; Lymphocyte and monocyte chemoattractant; LMC; Monotactin-1; MTN-1; NCC-4; Small-inducible cytokine A16; CCL16; ILINCK; NCC4; SCYA16

SAP130

79595

Q9H0E3

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human SAP130 recombinant protein (Position: H309-H913) .

Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma- activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.

Flow Cytometry

Angiogenesis, Cardiovascular, Chemokines, Cytokines, Immunology, Innate Immunity, Macrophage/Inflammation

Immunogen affinity purified.

Liquid

Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.

1. Fleischer, T. C., Yun, U. J., Ayer, D. E. Identification and characterization of three new components of the mSin3A corepressor complex. Molec. Cell Biol. 23: 3456-3467, 2003. 2. Metselaar, P. I., Hos, C., Welting, O., Bosch, J. A., Kraneveld, A. D., de Jonge, W. J., Te Velde, A. A. Ambiguity about splicing factor 3b subunit 3 (SF3B3) and Sin3A associated protein 130 (SAP30) . Cells 10: 590, 2021. 3. Seifert, L., Werba, G., Tiwari, S., Giao Ly, N. N., Alothman, S., Alqunaibit, D., Avanzi, A., Barilla, R., Daley, D., Greco, S. H., Torres-Hernandez, A., Pergamo, M., and 10 others. The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression. Nature 532: 245-249, 2016. Note: Erratum: Nature 591: E28, 2021.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

157578 MW

6

C-C motif chemokine ligand 16

Secreted.

BAG family molecular chaperone regulator 5

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.