Anti-tol-achtige receptor 4 TLR4 Antibody Picoband® fluoro647 Vervoeging

TLR4, Toll-like receptor 4, is a protein that in humans is encoded by the TLR4 gene. TLR 4 is a toll-like receptor. TLR4, the human homolog of Drosophila Toll, is a type I transmembrane protein with an extracellular domain consisting of a leucine-rich repeat region and an intracellular domain homologous to that of human interleukin-1 receptor. The TLR4 gene is mapped to chromosome 9q32-q33 by fluorescence in situ hybridization.It detects lipopolysaccharide from Gram-negative bacteria and is thus important in the activation of the innate immune system.The protein encoded by this gene is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.

Toll-like receptor 4; hToll; CD284; TLR4

TLR4

7099

O00206

Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the N-terminus of human TLR4.

Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.

Flow Cytometry

Adaptive Immunity, Atherosclerosis, Cardiovascular, Host-Virus Interaction, Immunoglobulins, Immunology, Innate Immunity, Interspecies Interaction, Macrophage/Inflammation, Microbiology, Receptors, TLR Signaling, Vascular Inflammation

Immunogen affinity purified.

Liquid

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) . Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS- independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni (2+) . Responses triggered by Ni (2+) require non-conserved histidines and are, therefore, species- specific. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. .

1. Medzhitov, R., Preston-Hurlburt, P., Janeway, C. A., Jr.A human homologue of the Drosophila Toll protein signals activation of adaptive immunity.Nature 388: 394-397, 1997. 2. Park, B. S., Song, D. H., Kim, H. M., Choi, B.-S., Lee, H., Lee, J.-O.The structural basis of lipopolysaccharide recognition by the TLR4-MD-2 complex.Nature 458: 1191-1195, 2009. 3. Rock, F. L., Hardiman, G., Timans, J. C., Kastelein, R. A., Bazan, J. F.A family of human receptors structurally related to Drosophila Toll.Proc. Nat. Acad. Sci. 95: 588-593, 1998.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

95680 MW

No cross reactivity with other proteins.

6

Toll-like receptor 4

Cell membrane; Single-pass type I membrane protein. Upon complex formation with CD36 and TLR6, internalized through dynamin-dependent endocytosis. .

Belongs to the Toll-like receptor family.

Toll-like receptor 4

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.