Anti-topo-isosomerase II-alfa/TOP2A Antibody Picoband® fluoro550 Vervoeging

The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22. The TOP2A gene spans approximately 30 kb and contains 35 exons. Furthermore, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes. Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, catenation, decatenation, knotting, and unknotting of circular DNA. It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments. There are about 100,000 molecules of topoisomerase II per HeLa cell nucleus, constituting about 0.1% of the nuclear extract. DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation. Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, but did not affect transcription on naked templates. Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II.

DNA topoisomerase 2-alpha;5.99.1.3; DNA topoisomerase II, alpha isozyme; TOP2A; TOP2

TOP2A

7153

P11388

Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human Topoisomerase II alpha, different from the related rat sequence by three amino acids, and different from the related mouse sequence by four amino acids.

Flow Cytometry

Cancer, Chromosome Structure, DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling

Immunogen affinity purified.

Liquid

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity) . .

1. Miller, K. G.; Liu, L. F.; Englund, P. T. : A homogeneous type II DNA topoisomerase from HeLa cell nuclei. J. Biol. Chem. 256: 9334-9339, 1981. 2. Mondal, N.; Parvin, J. D. : DNA topoisomerase II-alpha is required for RNA polymerase II transcription on chromatin templates. Nature 413: 435-438, 2001.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

174385 MW

No cross reactivity with other proteins.

6

DNA topoisomerase 2-alpha

Cytoplasm . Nucleus, nucleoplasm . Generally located in the nucleoplasm.

Belongs to the type II topoisomerase family.

DNA topoisomerase 2-alpha

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.