Anti-VEGFR-2 (Tyr-801) [geconserveerde site], Fosfospecifiek antilichaam

Vascular endothelial growth factor receptor-2 (VEGFR-2/Flk-1/KDR) is the primary receptor for VEGF in endothelial cells. Other VEGFR family members, VEGFR-1 (Flt-1) and VEGFR-3 (Flt-4), can also transduce the intracellular signals of VEGF. However, the role of VEGFR-1 is observed mainly during embryonic angiogenesis and VEGFR-3 signaling may be restricted to specific types of endothelial cells. Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr-951/996) and in the tyrosine kinase catalytic domain (Tyr-1054/1059) . Other sites, Tyr-1175 and Tyr-1212 provide docking sites for downstream signaling molecules. Activation of VEGFR-2 also phosphorylates Tyr-801, leading to PI3-kinase-Akt activation and increases in endothelial nitric oxide synthase activity. Phosphorylation of mutliple sites in VEGFR-2 is required for downstream activation of several signaling pathways that control proliferation, chemotaxis, and sprouting during angiogenesis.

Our VEGFR-2 (Tyr-801) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat VEGFR-2 (Tyr-801) and is antigen affinity purified. It is great for use in ELISA, WB, ICC.

Our VEGFR-2 (Tyr-801) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat VEGFR-2 (Tyr-801) and is antigen affinity purified. It is great for use in WB, ICC.

KDR, flk-1, Vascular endothelial growth factor receptor 2

KDR

P35968

Tyr-801

Human, Mouse, and Rat

Phospho-VEGFR-2 (Tyr-801) peptide (coupled to carrier protein) corresponding to amino acids surrounding Tyr-801 in human VEGFR-2. This sequence has high homology to the conserved site in rat and mouse VEGFR-2, and has significant homology to the conserved sites in VEGFR-1 (Tyr-794) and VEGFR-3 (Tyr-812) .

VEGFR-2 (Tyr-801)

Unconjugated

ELISA, WB, ICC

Cardiovascular, Cell Signaling, Growth Factor Signaling, Oncoproteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Phosphorylated

180

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:1000

The antibody detects a 180 kDa* band corresponding to VEGFR-2 in western blots of human endothelial cells treated with pervanadate, and shows strong reactivity toward recombinant human VEGFR-2. This reactivity is removed by akaline phosphatase treatment. In addition, the antibody detects the conserved site in VEGFR-1 (Tyr-794) . The antibody also works for immunofluorescent staining of VEGFR-2 in HUVEC cells.

1:100

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_2132330