Antiprioneiwit PrP/PRNP Antibody Picoband® fluoro550 Vervoeging

PRNP (prion protein), also known as CD230 and PRP, is a protein that in humans is encoded by the PRNP gene. The major prion protein is expressed in the brain and several other tissues. Expression is most predominant in the nervous system but occurs in many other tissues throughout the body. Puckett et al. (1991) identified a RFLP with a high degree of heterozygosity in the 5-prime region of the PRNP gene, which might serve as a useful marker for the pter-p12 region of chromosome 20. PRNP is associated with a variety of cognitive deficiencies and neurodegenerative diseases such as Creutzfeldt-Jakob disease, bovine spongiform encephalopathy, and kuru. PRNP is highly conserved through mammals, lending credence to application of conclusions from test animals such as mice. Comparison between primates is especially similar, ranging from 92.9-99.6% similarity in amino acid sequences.

Major prion protein; PrP; PrP27-30; PrP33-35C; CD230; Prnp; Prn-p, Prp

PRNP

19122

P04925

Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence in the middle region of mouse PRNP, identical to the related rat sequence.

Highly expressed in the brain, lung, kidney and heart. Expressed at low levels in the liver and spleen. .

Flow Cytometry

Hematopoietic Progenitors, Neurodegenerative Disease, Neurology Process, Neuroscience, Stem Cells, Surface Molecules

Immunogen affinity purified.

Liquid

May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity) . Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu (2+) or ZN (2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains. .

1. Bounhar, Y., Zhang, Y., Goodyer, C. G., LeBlanc, A.Prion protein protects human neurons against Bax-mediated apoptosis.J. Biol. Chem. 276: 39145-39149, 2001. 2. Brown, P., Galvez, S., Goldfarb, L. G., Nieto, A., Cartier, L., Gibbs, C. J., Jr., Gajdusek, D. C.Familial Creutzfeldt-Jakob disease in Chile is associated with the codon 200 mutation of the PRNP amyloid precursor gene on chromosome 20.J. Neurol. Sci. 112: 65-67, 1992. 3. Puckett, C., Concannon, P., Casey, C., Hood, L.Genomic structure of the human prion protein gene.Am. J. Hum. Genet. 49: 320-329, 1991.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

27977 MW

No cross reactivity with other proteins.

6

Major prion protein

Cell membrane ; Lipid-anchor, GPI-anchor . Golgi apparatus . Targeted to lipid rafts via association with the heparan sulfate chains of GPC1. Colocates, in the presence of CU (2+), to vesicles in para- and perinuclear regions, where both proteins undergo internalization. Heparin displaces PRNP from lipid rafts and promotes endocytosis. .

Major prion protein (PrP)

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.