APH1A Antibody / Gamma-secretase subunit APH-1A

Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Western Blot: 0.25-0.5 µg/mL

Q96BI3

Human, Mouse, Rat

A synthetic peptide corresponding to a sequence at the C-terminus of human APH1A was used as the immunogen for the APH1A antibody.

FACS, IF, IHC, WB

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This APH1A antibody is available for research use only.

After reconstitution, the APH1A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the APH1A antibody should be determined by the researcher.

Cytoplasm (ER, Golgi)

Immunofluorescent staining of APH1A using anti-APH1A antibody (red) . APH1A was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/ml rabbit anti-APH1A antibody overnight at 4oC. DyLight 550 Conjugated Donkey Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.