BACE1 Proteïne (C-His, Mouse)

BACE1

ASP2, Bace, Bace1

C-His

< 1.0 EU per μg of the protein as determined by the LAL method

≥ 90 % as determined by SDS-PAGE

Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys (Dpn) -Arg-Arg-NH2 (Catalog# ES004, R&D Systems) . Cleavage of ES004 can be measured using excitation and emission wavelengths of 320 and 405 nm, respectively. The specific activity is >2 pmoles/min/μg.

Met1-Thr457

The secreted recombinant mouse BACE1 consists of 447 amino acids and has a calculated molecular mass of 49.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 60-65 kDa protein in SDS-PAGE under reducing conditions.

Mouse