CD20 Antilichaam

This antibody recognizes a protein of 33-37kDa, identified as CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

P11836

Human

Human tonsil B cells were used as the immunogen for this CD20 antibody.

SPM494

IHC-P

Bioreactor concentrate

1X PBS, pH 7.4

This CD20 antibody is available for research use only.

CD20 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Predominantly cell surface with some cytoplasmic

IHC testing of CD20 antibody and FFPE human lymphoma tissue (clone SPM494) .