CD276 Proteïne (C-His, Human)

CD276

4Ig-B7-H3, B7-H3, B7H3, B7RP-2, PSEC0249, UNQ309/PRO352

C-His

< 1.0 EU per μg of the protein as determined by the LAL method

≥ 90 % as determined by SDS-PAGE.

1.Flow cytometric analysis of anti-B7-H3 CAR expression. 5e5 of anti-B7-H3 CAR-293 cells were stained with 100 μL of 4 μg/ml of AF 488-conjugated human B7-H3 Protein (ECD, His Tag, Site-Specific AF488 Conjugation, Cat. No. 11188-H86H-SD) and negative control protein. Non-transduced 293 cells were used as a control (left) . AF 488 signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of PE-conjugated B7-H3 protein to PBMC cells. PBMC cells were stained with anti-CD3 antibody and AF 488-conjugated human B7-H3 protein (Site-Specific Conjugation, ECD, His Tag, Cat. No. 11188-H86H-SD) and detected by flow cytometry. PBMC cells stained with anti-CD3 antibody were used as a control (left) (QC tested) .

Met1-Thr461

The recombinant human B7-H3 consists of AA Met1-Thr461 with a polyhistidine tag at the C-terminus. It has a predicted molecular mass of 50 kDa.

Human