CD28 / TP44 Proteïne (Fc-tag)

CD28 (Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

Cd28 Protein

NP_031668.3

Mouse

Mouse

HFc-Tag

See Datasheet send with the product

< 1.0 EU per μg protein as determined by the LAL method.

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS.

1.Loaded Recombinant Mouse CD28 Protein on proA Biosensor, can bind Recombinant Mouse B7-1/CD80 Protein with an affinity constant of 1.410 μM as determined in BLI assay (Sartorius Octet RED384) . 2.Loaded Recombinant Mouse CD28 Protein on proA Biosensor, can bind Recombinant Mouse B7-2/CD86 Protein with an affinity constant of 7.750 μM as determined in BLI assay (Sartorius Octet RED384) .

368

Lyophilized

PBS, pH 7.4

See Datasheet

119.2 kDa (glycosylated)

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

HEK 293 cells