CHFR Antilichaam - N-terminalgebied: FITC (ARP43250_P050-FITC)

Checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase

RNF116, RNF196

55743

Q96EP1

NP_060693

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

The immunogen is a synthetic peptide directed towards the N terminal region of human CHFR

CHFR is an E3 ubiquitin-protein ligase required to transiently arrest cells in early prophase when they are exposed to microtubule poisons. It acts in early prophase before chromosome condensation, when the centrosome moves apart from each other along the periphery of the nucleus. CHFR probably promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. This suggests that it may be involved in signaling the presence of mitotic stress caused by microtubule poisons.

USP7; VIM; UBE2D2; AURKA; HLTF; RANBP2; PLK1; PCNA; MCM2; HDAC2; HDAC1; CDK5; CDC20; PARP1; SIN3A; CHFR; UBC13; SUMO1; UBE2I; LRP1; UBE2V1; UBE2N; UBE2D1; TRIM9; RNF125; CBLC; RNF7; RELA; RNF8; HIST2H2BE; HIST2H2AC; UBC; SMARCD1; SMARCB1; SMARCA4; KIF22

FITC: Fluorescein Isothiocyanate

Polyclonal Antibody

IHC, WB

Affinity Purified

CHFR is supported by BioGPS gene expression data to be expressed in HepG2

0.5 mg/ml

Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%

Liquid. Purified antibody supplied in 1x PBS buffer.

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

69kDa

Kang, H.C., Cancer Lett. 260 (1-2), 170-179 (2008)

Wet Ice

623

CHFR

CHFR

Rabbit

E3 ubiquitin-protein ligase CHFR

NM_018223

REWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVIN