DKK1 Proteïne (C-His, Human)

DKK1

DKK-1, SK, UNQ492/PRO1008

C-His

< 10 EU per mg protein.

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

1.Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.1-0.4 μg/ml in the presence of 10 ng/mL of mouse Wnt3a. 2.Loaded Recombinant Human LRP-6 Protein, His & AVI Tag, Avi-tag Biotinylated (Cat. No. 11968-H49H-B) on SA Biosensor, can bind Recombinant Human DKK-1 Protein, His Tag (Cat. No. 10170-H08H) with an affinity constant of 90.6 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Met2-His266

The recombinant human DKK1 protein consists of 246 amino acids and has a calculated molecular mass of 27.22 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh DKK1 is approximately 42 kDa due to glycosylation.

Human