GARS sf9 Recombinant eiwit

Source : Sf9 Insect Cells. GARS Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 78,902 Dalton. GARS is expressed with a -10xHis tag at N-terminus and purified by proprietary chromatographic techniques. GARS is an (alpha) 2 dimer which is a member of the class II family of tRNA synthetases. GARS is a glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases which charge tRNAs with their cognate amino acids. GARS catalyzes the attachment of glycine to tRNA (Gly) . In addition, GARS is able to produce diadenosine tetraphosphate (Ap4A), which is a universal pleiotropic signaling molecule required for cell regulation pathways, by direct condensation of two ATPs. GARS has been demonstrated to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.

Glycine--tRNA ligase||EC 6.1.1.14||Diadenosine tetraphosphate synthetase||AP-4-A synthetase||Glycyl-tRNA synthetase||GlyRS||GARS||HMN5||CMT2D||DSMAV||SMAD1.

Greater than 80.0% as determined by SDS-PAGE.

GARS is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.