IL-2 (menselijk) (Recombinant) (niet vermeld)

Source :E. coli Specific Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells, and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils, and induces mononuclear cells to secrete IFN-gamma and TNF-alpha and -beta. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important hemeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens.

Interleukin-2; Aldesleukin

Human

Functional Assay

>=95% (SDS-PAGE)

Reconstitute 10μg vial with 100 μl sterile water to a concentration of 0.1mg/mL. Reconstitute 50μg vial with 100 μl sterile water to a concentration of 0.5mg/mL. Further dilute in other aqueous solutions. Lyophilized from sterile 0.1% TFA, 70% acetonitrile.

Short Term Storage +4°C ; Long Term Storage-20°C ; Avoid freeze/thaw cycles. Stable for at least 1 year after receipt when stored at -80°C. Working aliquots are stable for up to 3 months when stored at -20°C.

MW :~14kDa (SDS-PAGE) Biological Activity: Measured in a cell proliferation assay using CTLL2 cells. The ED50 for this effect is typically 0.35-1.4ng/ml. Endotoxin Content: <0.01EU/μg protein (LAL test; Lonza) .

The extracellular domain of human IL-2 (aa 21-153) with a N-terminal Met.