LH1 Antilichaam / PLOD1

Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.

Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:10-1:5, Flow cytometry: 1:25 (1x10e6 cells)

Q02809

Human

A portion of amino acids 66-94 from the human protein were used as the immunogen for the LH1 antibody.

WB, IHC-P, FACS

Antigen affinity purified

Purified

This LH1 antibody is available for research use only.

Aliquot the LH1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

In 1X PBS, pH 7.4, with 0.09% sodium azide

The stated application concentrations are suggested starting points. Titration of the LH1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Western blot testing of human 1) A431, 2) MCF7, 3) U-2 OS and 4) U-87 MG cell lysate with LH1 antibody. Predicted molecular weight ~83 kDa.