Monoklonale antilichamen tegen biotine (vitamine B7 of vitamine H) (kloon: SPM375)

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

All species

Biotinylated sheep immunoglobulin

Biotin (Vitamin B7 or Vitamin H)

SPM375

Confocal Microscopy, FACS, IF, WB, IHC

Affinity Chromatography

Purified

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Confocal Microscopy; Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; In situ Nucleic Acid Hybridization; Western Blot; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)

Mouse IgG1, kappa