Monoklonale antilichamen tegen cytokeratine, zuur (type I of LMW) (epitheliaal marker) (kloon: SPM115)

This MAb recognizes the 56.5kDa (CK10) ; 50kDa (CK14) ; 50kDa (CK15) ; 48kDa (CK16) ; 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. MAb SPM116 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

KRT77||KRT1B

KRT77

374454

Q7Z794

Human, Mouse, Rat, Turtle

Human epidermal keRatin

Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker)

SPM115

FACS, IF, WB, IHC

Affinity Chromatography

Purified

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)

Mouse IgG1, kappa