Monoklonale antilichamen tegen mitochondria (Marker voor menselijke cellen) (Kloon: MTC754)

This MAb recognizes a 60kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in cells from a wide variety of animals including insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Human

Mitochondrial fraction of HeLa cells

Mitochondria (Marker for Human Cells)

MTC754

FACS, IF, WB, IHC

Affinity Chromatography

Purified

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Western Blot (0.25-0.5ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA Buffer, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)

Mouse IgG1, kappa