NNT-1/BCSF-3

NNT-1/BCSF-3 is a neurotrophic factor with B-cell stimulating capabilities. Expressed in lymph nodes and spleen, NNT-1/BCSF-3 binds and activates glycoprotein 130 (gp130) and leukemia inhibitory factor receptor member beta (LIFR-beta) and induces tyrosine phosphorylation of these receptors. In vitro, it supports the survival of chick embryo motor and sympathetic neurons. In mice, NNT-1/BCSF-3 induces serum amyloid A, causes body weight loss and B cell hyperplasia associated with increased in serum IgG and IgM. Recombinant human NNT-1/BCSF-3 is a 22.4 kDa protein containing 199 amino acid residues.

CLCF1; CLC; NR6; BSF3; NNT1; BSF-3; CISS2; NNT-1

23529

Q9UBD9

NP_037378.1

NM_013246

11q13.3

Human

Chicken

MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF

< 0.1 ng/µg of protein (< 1EU/µg)

> 98% by SDS-PAGE & HPLC analyses

NT-1/BCSF-3 weakly supports chick E8 DRG neurite outgrowth at a concentration of 1.0 ng/ml.

199

Lyophilized

22.4 kDa

E. coli