Periostin-antilichaam/POSTN

Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for AlphaVbeta3 and AlphaVbeta5 integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor beta 1 (TGF beta 1) . Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain.

Immunohistochemistry (FFPE) : 1-2 µg/mL

Q15063

Human

A portion of amino acids 193-326 from human POSTN protein was used as the immunogen for the Periostin antibody.

POSTN/4096

IHC-P

Protein G affinity

Purified

This Periostin antibody is available for research use only.

Aliquot the Periostin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the Periostin antibody should be determined by the researcher.

Secreted, Cytoplasm

IHC staining of FFPE human adrenal gland tissue with Periostin antibody (clone POSTN/4096) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.