Recombinant Gastrin Antilichaam

Gastrin, is a hormone that normally formed by mucosal cells in the gastric antrum and by the D cells of the pancreatic islets. Its primary function is to stimulate secretion of HCl by the gastric mucosa. HCl, in turn, inhibits gastrin formation. It also responsible for stimulating smooth muscle contraction and increasing blood circulation and water secretion in the stomach and intestine. Gastrin is regulated by epidermal growth factor in both mice and humans. Gastrin is excreted in excess by pancreatic tumors in the Zollinger-Ellison syndrome. Gastrin-Releasing Peptide (GRP) stimulates the release of gastrin as well as other gastrointestinal hormones and also acts as an autocrine growth factor for certain cell types. High levels of GRP are found in the human lung just after birth and levels decrease thereafter in parallel with the observed disease in a number of pulmonary neuroendocrine cells. GRP is known to promote lung tumorigenesis in model systems.

Immunohistochemistry (FFPE) : 1-2 µg/mL

P01350

Human

A portion of amino acids 1-101 from human GAST protein was used as the immunogen for the recombinant Gastrin antibody.

GAST/8646R

IHC-P

Protein A affinity

Purified

This recombinant Gastrin antibody is available for research use only.

Aliquot the recombinant Gastrin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the recombinant Gastrin antibody should be determined by the researcher.

Cytoplasm, Secreted

IHC staining of FFPE human gastric antrum tissue with recombinant Gastrin antibody (clone GAST/8646R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.