Recombinant humaan TIE2/TEC eiwit

TEK, also known as angiopoietin-1 receptor isoform 1, is an endothelial cell-specific receptor tyrosine kinase subfamily (RTK) . This protein acts as cell-surface receptor and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton. It has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels and is required for normal angiogenesis and heart development during embryogenesis. Recombinant human TEK protein, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human Angiopoietin-2 (CAT# ATGP3853) is coated at 5 µg/mL (100 ul/well) can bind Human TIE-2. The ED50 range ≤1 µg/mL.

TEK, TEK receptor tyrosine kinase, VMCM, venous malformations, multiple cutaneous and mucosal, TEK tyrosine kinase, endothelial, TIE2, TIE-2, VMCM1, CD202b, angiopoietin-1 receptor, Hyk, AA517024, STK1, p140 TEK

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human Angiopoietin-2 (CAT# ATGP3853) . The ED50 range ≤1 µg/mL.

107.9 kDa (965aa)

Tie2, Angiopoietin-1 receptor isoform 1, TEK, CD202B, GLC3E, TIE-2, TIE2, VMCM, VMCM1, ATGP3947-10 µg, ATGP3947-20 µg, ATGP3947-50 µg, ATGP3947-100 µg, ATGP3947-250 µg, ATGP3947-500 µg, ATGP3947-1 mg, ATGP3947-010, ATGP3947-020, ATGP3947-050, ATGP3947-100, ATGP3947-250, ATGP3947-500, ATGP3947-01M, ATGP3762

Kitajima D., et al, (2016) J Cancer. 7:600-607.; ; Kelly-Goss MR., et al, (2017) Sci Rep. 7:9049.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_000450

Q02763

Human

Ala23