Recombinant Soja Bean P34 Proteïne, GST Tag

Source : Escherichia Coli. The E.Coli derived GST Tag recombinant protein, 36kDa, contains Soybean P34 Protein epitopes 214-261, 351-379 amino acids. The P34 protein is the main allergen for soybean sensitive humans. Soybean protein P34, a thiol protease belonging to the papain family, is a monomeric allergen having an N-terminal amino acid sequence and amino acid composition identical to that of the seed 34kDa protein. It is an insoluble glycoprotein having a pI of 4.5 and a calculated mass of 28.643 Dalton, representing 2-3% of total soybean protein. Upon glycosylation, the mass will be somewhat larger, resulting in a ~32kDa band in non-reduced SDS PAGE gels. It exhibits no enzymatic function due to an absence of the catalytic cysteine. P34 is stored in storage vacuoles of soybean cotyledons.

Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

50mM Tris-HCl, pH 8.0, 60mM NaCl, 10mM glutathione and 50% glycerol.

Soybean P34 His although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.