SHD Antibody / SH2 domein-bevattende adapter eiwit D

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q96IW2

Human, Mouse, Rat

E.coli-derived human SHD recombinant protein (Position: M1-P340) was used as the immunogen for the SHD antibody.

WB, IHC, IF, FACS, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This SHD antibody is available for research use only.

After reconstitution, the SHD antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the SHD antibody should be determined by the researcher.

Cytoplasmic

Immunofluorescent staining of SHD using anti-SHD antibody (red) . SHD was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/ml rabbit anti-SHD antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.