Zebrafish Eno1 Antilichaam / Eno1a / Eno1b

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q6PC89, A0A2R8Q1X2

Zebrafish

A synthetic peptide corresponding to a sequence in the middle region of zebrafish ENO1a/b was used as the immunogen for the Zebrafish Eno1 antibody. This antibody will detect the a and b isoforms.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Eno1 antibody is available for research use only.

After reconstitution, the Zebrafish Eno1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Eno1 antibody should be determined by the researcher.

Cytoplasm

IHC staining of FFPE zebrafish kidney tissue with Zebrafish Eno1 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.