Humaan IRF3-antilichaam

Members of the Interferon regulatory factor (IRF) family regulate gene expression critical to immune response, hemopoiesis, and proliferation. IRF-3 is a member of the IRF family, and is distinct from other familymembers. Its transcriptional activity is regulated solely by posttranslational modifications. It plays a crucialrole in activation of innate immunity and inflammation in response to viral infection.

IRF3, Interferon regulatory factor-3, IRF-3, Interferon regulatory factor 3, IRF 3

Human

Human

Recombinant human IRF-3 (108-166aa) purified from E. coli

3F10

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

IRF3, ATGA0560-10 µg, ATGA0560-20 µg, ATGA0560-50 µg, ATGA0560-100 µg, ATGA0560-250 µg, ATGA0560-500 µg, ATGA0560-1 mg, ATGA0560-10, ATGA0560-20, ATGA0560-50, ATGA0560-100, ATGA0560-250, ATGA0560-500, ATGA0560-1

DeFilippis VR et al., (2006) J Virol 80 (2) :1032-1037.; ; Pietila TE et al., (2007) J Immunol 178 (1) :253-261.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenetics and Nuclear Signaling

NP_001562

Q14653

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: MCF7 cell lysate; ; Lane 2.: K562 cell lysate; ; {2004010499158921.jpg}

Flow cytometry analysis of IRF3 in MCF7 cells. The cell was stained with ATGA0560 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .Flow cytometry analysis of IRF3 in Jurkat cells. The cell was stained with ATGA0560 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .{2004010499231937.jpg}

ICC/IF analysis of IRF3 in HeLa cells. The cell was stained with ATGA0560 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .