Recombinant humaan Glutathion S-transferase alfa-4-GSTA4-eiwit

GSTA4, also known as glutathione S-transferase A4, belongs to the GST superfamily. This enzyme is involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. GSTA4 shows very high activity with reactive carbonyl compounds such as alk-2-enals. GSTA4 is highly effective in catalyzing the conjugate addition of reduced glutathione to 4-hydroxynonenal, an important product of peroxidative degradation of arachidonic acid and a commonly used biomarker for oxidative damage in tissue. This enzyme is expressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver. Recombinant human GSTA4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 4,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Glutathione S-transferase alpha 4, GSTA4-4, GTA4

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 4,000pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

28.3 kDa (246aa) confirmed by MALDI-TOF

GSTA4, Glutathione S-transferase alpha 4, GSTA4-4, GTA4, ATGP3141-10 µg, ATGP3141-20 µg, ATGP3141-50 µg, ATGP3141-100 µg, ATGP3141-250 µg, ATGP3141-500 µg, ATGP3141-1 mg, ATGP3141-10, ATGP3141-20, ATGP3141-50, ATGP3141-100, ATGP3141-250, ATGP3141-500, ATGP3141-1

Bruns C.M., et al. (1999) J. Mol. Biol. 288:427-439; ; Balogh L.M., et al. (2010) Biochemistry 49:1541-1548.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) 2mM DTT, 20% glycerol,100 mM NaCl

Metabolism

NP_001503

O15217

Human

MGSSHHHHHH SSGLVPRGSH MGSHMAARPK LHYPNGRGRM ESVRWVLAAA GVEFDEEFLE TKEQLYKLQD GNHLLFQQVP MVEIDGMKLV QTRSILHYIA DKHNLFGKNL KERTLIDMYV EGTLDLLELL IMHPFLKPDD QQKEVVNMAQ KAIIRYFPVF EKILRGHGQS FLVGNQLSLA DVILLQTILA LEEKIPNILS AFPFLQEYTV KLSNIPTIKR FLEPGSKKKP PPDEIYVRTV YNIFRP